Fig. 8
From: Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions
TSP-1 depletion reduces the synaptogenic effects of C57ACM. a TSP-1 was immunodepleted from crude C57ACM or from the ≥10 kDa fraction and depletion validated by immunoblotting. Blot showsTSP-1 amounts in input, fraction bound to beads, and eluted supernatant after magnetic separation. b Representative pictures of SNP and NeuN immunocytochemistry. Complete ACM and TSP-1-depleted ACM were added to the neuronal cultures for 8 days after which cultures were fixed and immunolabelled with antibodies against SNP and NeuN. c Quantification of SNP intensity. For quantification of staining intensity of SNP in the green channel from three slides for each experimental condition were analysed. Results indicate a mean which corresponds in each case to the mean of four fields. *p < 0.05 for these comparisons: C57N + C57ACM vs C57N + C57ACM-TSP-1; P301SN + C57ACM vs P301SN + C57ACM-TSP-1. Values were analysed using Tukey’s multiple comparisons test. ANOVA revealed no interaction between genotype and culture condition (ACM or ACM-TSP-1) [F (1, 12) = 0.9814; p = 0.3414] but a significant effect was found for genotype [F (1, 12) = 62.94; P < 0.0001] while no effect for culture type [F (1, 12) = 1.476; p = 0.2478]