Fig. 7
From: Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions
TSP-1 expression is reduced in the superficial cortex and in cultured astrocytes or ACM from P301S mice. a Lysates from the superficial cortex of 3 and 5 month-old mice or b from astrocytes from 98% pure cultures from 7 day-old mice were analysed for TSP-1 expression by immunoblotting. c Quantitative analysis of TSP-1 expression normalised to actin. Values for C57-derived controls are set to 1. *p < 0.05 for these comparisons: C57 3 m vs P301S 3 m; C57 5 m vs P301S 5 mo; C57A vs P301SA. d Analysis of ACM from C57A or P301SA after fractionation on an Amicon filter with a 10 kDa cutoff. A significant decrease in TSP-1 was observed in P301SACM compared to C57ACM, *p < 0.05; e Representative blot, and f quantification of TSP-1.expression in C57ACM, pure C57 neuronal cultures (C57NCM), and C57 neurons co-cultured with C57ACM or P301SACM. Ponceau S staining of ACM blot to show equal loading. *p < 0.05 for these comparisons: C57N + C57ACM vs C57N + P301SACM. C57A released more TSP-1 than C57N, C57A vs C57N. The data represent a mean of three independent experiments performed in triplicates; mean ± S.E.M, statistical analyses performed with Mann–Whitney test