Fig. 5
From: Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions
ACM from P301SA reduces synaptic protein expression in cultured neurons. C57N or P301SN cultures were exposed to C57ACM or P301SACM for 8 days after which cell lysates were analysed by immunoblotting. a Representative immunoblot of synaptophysin (SNP) and PSD95 in neuronal cultures with and without ACM exposure. Note the significant decline of both (b) SNP and (c) PSD95 when C57N or P301SN were cultured with P301SACM compared to neurons maintained in C57ACM. Data were normalised to β actin and represent mean ± SEM of three independent experiments performed in triplicate. *p < 0.05 for these comparisons: C57N vs C57N + P301SACM; C57N + P301SACM vs C57N + C57ACM; P301SN vs P301SN + P301SACM; P301SN vs P301SN + C57ACM; Tukey’s multiple comparisons test both for SNP and PSD95. ANOVA for SNP values revealed a significant interaction between genotype and culture condition [F (2, 12) = 29.88; p = 0.0001], significant effect of genotype [F (1, 12) = 307.2; p = 0.0001] and significant effect of culture treatment [F (2, 12) = 34.68; p = 0.0001]. ANOVA for PSD95 values revealed a significant interaction between genotype and culture condition (ACM) [F (2, 12) = 18.08; p = 0.0002], significant effect of genotype [F (1, 12) = 112.2; p = 0.0001] and culture treatments [F (2, 12) = 37.01; p = 0.0001]