Fig. 1

Astrocytes from P301S mice express more GFAP and S100β and less GS, GLT-1 and GLAST than astrocytes from control mice. a, d Representative blots of astrocyte-specific protein markers related to gliosis/proliferation (GFAP, S100β) and function (glutamine synthetase (GS) and the glutamate transporters (GLT-1 and GLAST)) in the superficial motor cortex of 3 month-old and 5 month-old C57 and P301S mice. h Expression of the same markers in primary pure cultured astrocytes from 7 day-old mice after days 8 in vitro (98% culture confluence). Mean ± SEM, *p < 0.05 vs control; unpaired t test, N = 3 independent experiments (mice: GFAP, S100β (b, c); GLT-1, GLAST, GS (e–g); primary cultures i). Vertical lines in (d) denote point were the picture of the Western blot was assembled from two parts cut from the same blot