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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Distinct deposition of amyloid-β species in brains with Alzheimer’s disease pathology visualized with MALDI imaging mass spectrometry

Fig. 1

MALDI-IMS for frozen AD brain sections. A: Aβ1–40 deposits in the leptomeningeal blood vessels and arterioles (red) and Aβ1–42 deposits in cerebral parenchyma (green). The m/z 4939.9 was used to detect the tissue structure and shows an unknown biomolecule (blue). B: Optical density for MALDI-IMS. This figure is a magnification of the region within the dotted square in Fig. 1A. Aβ1–40 is deposited in leptomeningeal blood vessels (1 and 5) and arterioles (4) shown in red. Aβ1–42 is deposited in cerebral parenchyma as senile plaques (2 and 3) shown in green. C: MALDI Mass spectrum in leptomeningeal blood vessels (LMV), arterioles (Ao), and senile plaque (SP) of Fig. 1B. Aβ1–40 and N-terminal truncated Aβx-40 are located in Ao, while Aβ1–36 to Aβ1–41 are in LMV. Aβ1–42, Aβ1–43, and N-terminal truncated Aβx-42 are preferentially located in SP. D: MALDI-IMS and IHC of various C-terminal truncated Aβ peptides in AD with severe CAA. (a) MALDI-IMS 100 μm resolution imaging for Aβ1–40 (red) and Aβ 1–42 (green). (b) Highlight 20 μm resolution leptomeningeal blood vessels and cortex imaging in dotted square (a). (c) Highlight of an arteriole in solid square (b). Adjacent sections of the occipital cortex from AD brains were immunostained and focused on arteriole and cerebral parenchyma (c) using antibodies against Aβ40 (d: BA27) or Aβ42 (e: anti-Aβ42 polyclonal) and merged view (f). Both analyses demonstrated that Aβ40 is preferentially deposited in leptomeningeal blood vessels and arterioles in the subarachnoid space and the cerebral parenchyma forming CAA. In contrast, Aβ42 is mainly deposited in SP. IHC analysis also demonstrated the differential distribution of Aβ40 and Aβ42, which were CAA dominant and SP dominant deposition, respectively. Solid rectangles indicate the area illustrated in the panel. Scale bars = 100 μm. E: MALDI-IMS of various C-terminal truncated Aβ peptides in AD with severe CAA (NO. 3). Aβ1–36 to Aβ1–41 are preferentially deposited in leptomeningeal blood vessels, while Aβ1–42 and Aβ1–43 are deposited in the cerebral parenchyma as senile plaques

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