Fig. 8

Cln3 ^−/− Astrocytes have a migration defect. Wild type (WT) and Cln3-deficient (Cln3 ^−/−) primary cortical astrocytes were plated on Essen Image Lock 24 well plates, grown to confluence then scratched using an Essen wound maker. a Representative pictures of the wound at three time points. b The distance migrated by WT and Cln3 ^−/− astrocytes every 4 h was calculated by comparing wound widths between the start and the different time points. WT astrocytes migrated significantly further than did Cln3 ^−/− astrocytes. c The rate of migration was measured by calculating the distance migrated by these cells/h. Cln3 ^−/− astrocytes migrated significantly slower than WT astrocytes. In each experiment three wound widths were measured per well and three wells quantified per experiment