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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Glial cells are functionally impaired in juvenile neuronal ceroid lipofuscinosis and detrimental to neurons

Fig. 2

Attenuated morphological transformation of Cln3 −/− microglia. The morphology of wild type (WT) and Cln3-deficient (Cln3 −/−) microglia studied under basal conditions and after stimulation with LPS was revealed by CD68 immunostaining (red). A Cultures of unstimulated WT microglia were mainly bipolar but upon LPS stimulation for 24 h these cells rapidly changed shape. In contrast, while cultures of Cln3 −/− microglia exhibited a heterogeneous morphology and had intense immunostaining for CD68 under basal conditions these cells failed to dramatically change shape upon stimulation for 24 h. B To quantify morphological changes over time, cells were divided into three categories: Type 1 cells (resting microglia); Type 2 cells (migrating/activated microglia); type 3 (amoeboid activated microglia). C The transformation of type 1 cells into type 2 cells initially occurred more slowly in Cln3 −/− microglial cultures upon stimulation, with type 3 cells first appearing in cultures of both genotypes around 48 h regardless of treatment. Scale bars = 50 μm (A, B)

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