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Fig. 12 | Acta Neuropathologica Communications

Fig. 12

From: Glial cells are functionally impaired in juvenile neuronal ceroid lipofuscinosis and detrimental to neurons

Fig. 12

Cln3 −/− cells negatively impact WT cells. P0 cortical wild type (WT) and Cln3-deficient (Cln3 −/−) neuronal cultures were combined with either WT or Cln3 −/− mixed glia cultures to study the impact of these glial cells on neuronal health. A After 7 days of co-culture, WT co-cultures were healthy (a) but Cln3 −/− mixed glia appeared to have a detrimental effect when cultured with both WT (b) and Cln3 −/− neurons (d) with the latter being more dramatically affected. When WT mixed glia were co-cultured with Cln3 −/− neurons, neuronal survival improved. B Significantly less LDH released was observed in WT neuron/WT mixed glia co-cultures compared to Cln3 −/− neuron/Cln3 −/− mixed glia co-cultures. Scale bar in (A) = 20 μm

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