Fig. 8

Electrophysiological deficits in BFCNs from AD lines. a Co-localization of biocytin-labelled neurons (green) with cholinergic markers ChAT (red) and VAChT (blue). Arrows indicate positions of recorded neurons somas, scale bar is 50 μm. b Representative firing patterns of BFCNs produced by a 1 sec negative and positive square current injection are depicted. A grand total of 94 individual neurons were studied electrophysiologically: 22 wild-type control neurons, 21 familial control neurons, 18 AD1 neurons, 28 AD2 neurons, and 5 iAD1_ (CRISPR-corrected) neurons. The experiments on the 94 neurons required days to weeks. On each experimental day, representatives from each genotype were included, with at least three samples from each genotype studied each day. c Summary data on maximum number of action potentials that neurons are capable of sustaining (left) and height of a single action potential at rheobase (right) across all conditions. Individual data points are shown as circles, group means are shown as bars. **, p < 0.01 Tukey HSD test