Fig. 2

Basal cholinergic markers in PSEN2 ^N141I neuroprecursors. a Table showing the cell lines used. Four iPS lines reprogrammed from fibroblasts were used; two controls (949 and 050643, labelled as fControl and Control, respectively) that do not carry the PSEN2 ^N141I mutation nor the ε4 allele; and two AD patients (948 and 950, labelled as AD1 and AD2, respectively) who carry the mutation and the ε4 allele. Three of the four iPS lines were family related (fControl, AD1, and AD2). b Representative Sanger sequencing chromatograms showing a fragment of exon 5 of PSEN2. Red arrow marks site of the missense point mutation Chr1:227,073,304 A > T. c Immunocytochemistry and RT-PCR for early neuronal and basal forebrain markers. n = 3, 3 independent experiments with technical triplicates. d RTPCR fold changes for TUJ1 and BF1. n = 3, 3 independent experiments with technical triplicates. e Representative histograms for P75 staining. n > 6. f Aβ40 and Aβ42 ELISA quantifications. n = 3, 3 independent experiments with technical triplicates. ***, p < .001. *, p < .05