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Fig. 9 | Acta Neuropathologica Communications

Fig. 9

From: Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling

Fig. 9

Effects of blockers of voltage-gated calcium channels and inhibitors of ionotropic glutamate receptors on CNS SLE CSF #4 - induced calcium transients. a A representative trace of normalized fluorescence with gradually decreasing amplitudes following differential drug treatments to block action potentials (TTX), voltage-gated calcium channels (VGCCblock) and AMPA/kainate and NMDA receptors (GluRinh). b Summary histogram of peak amplitudes confirming that blockade of voltage-gated and ionotropic receptors decreases the amplitude of the control response. c Representative trace and d summary histogram of CNS SLE CSF #4 - induced calcium transients showing that GluRinh drugs alone lower the amplitude of the control response. e NMDA receptor blockade with 200 μM DL-AP5 completely abolished the control response, suggesting a predominant role for NMDA receptors in CNS SLE CSF-induced calcium transients. The addition of CSF in (a, c, e) is indicated by black dots below the trace. Drugs used are indicated by the gray timelines above the trace and calibration within the trace on the right. * p < .001, two-tailed paired t-test. Abbreviations: TTX – Tetrodotoxin; VGCCblock – GVIA + AGA + NIF; GVIA – ω-conotoxin GVIA; AGA – ω-agatoxin TK; NIF – Nifedipine; GluRinh – CNQX + D-AP5; CNQX – 6-Cyano-7-nitroquinoxaline-2,3-dione; D-AP5: D-2-Amino-5-phosphonopentanoic acid; DL-AP5 – DL-2-Amino-5-phosphonopentanoic acid

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