Fig. 10
Comparison of averaged intracellular calcium transients evoked by effective CNS SLE CSF and commercially available antibodies in time. a Trace illustrating that CNS SLE CSF #2 diluted to 1:10 evoked only a fast transient that peaked 3 s after CSF application. b The calcium response to 1:25 CNS SLE CSF #4 exhibited a fast transient after 3 s that was followed by a slower component measured 3 min after application and a return to basal levels after wash. c Anti-NR2A evoked a fast calcium transient that peaked at 6 s and a slow component that peaked after 3 min and could not be washed. d Anti-RPLP0 failed to induce a fast transient but provoked a slow and sustained rise of [Ca2+]i 3 min after application. The application of CSF/antibodies in (a–d) is indicated by an upward arrow, duration by the gray line above the trace, wash by a thick downward arrow, and depolarizing 5 s pulse (50 mM K+) by a black strip above the trace. The origin and dilution of CSF samples, as well as the antibodies and concentration used, are indicated above the gray line