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Fig. 10 | Acta Neuropathologica Communications

Fig. 10

From: Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling

Fig. 10

Comparison of averaged intracellular calcium transients evoked by effective CNS SLE CSF and commercially available antibodies in time. a Trace illustrating that CNS SLE CSF #2 diluted to 1:10 evoked only a fast transient that peaked 3 s after CSF application. b The calcium response to 1:25 CNS SLE CSF #4 exhibited a fast transient after 3 s that was followed by a slower component measured 3 min after application and a return to basal levels after wash. c Anti-NR2A evoked a fast calcium transient that peaked at 6 s and a slow component that peaked after 3 min and could not be washed. d Anti-RPLP0 failed to induce a fast transient but provoked a slow and sustained rise of [Ca2+]i 3 min after application. The application of CSF/antibodies in (ad) is indicated by an upward arrow, duration by the gray line above the trace, wash by a thick downward arrow, and depolarizing 5 s pulse (50 mM K+) by a black strip above the trace. The origin and dilution of CSF samples, as well as the antibodies and concentration used, are indicated above the gray line

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