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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Alzheimer’s disease pathological lesions activate the spleen tyrosine kinase

Fig. 8

The degree of colocalization of pSyk and tau differs for various tau epitopes. Sections from Tg Tau P301S mice (n = 4, 47 ± 3.1-week-old) were stained with antibodies against pTau (S202, S396/404, Y18), tau oligomers (TOC1) or tau conformers (MC1) and pSyk (Y525/526, green). The cortices were divided in ROIs (each at a size of 50,000μm2) and neurons singly immunopositive for pSyk, singly immunoreactive for the respective tau epitope and the neurons immunopositive for both pSyk and the respective tau epitope (colocalized) were counted and the percentages of each neuronal fraction calculated using Zen Blue 2.1 (Zeiss) and Excel (MS Office), respectively. In average, 509 cortical fields were analyzed for each epitope (total of 21,800 neurons counted). The percentage of neurons singly immunopositive for pSyk was at a similar level for all tau epitopes investigated (pSyk only). MC1 and pTau Y18 show the highest colocalization with pSyk whereas the incidence of neurons immunopositive for both pSyk and TOC1 or pTau S202 was much lower (colocalized fraction). The error bars represent SEM

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