Fig. 14

Syk overexpression increases tau phosphorylation and total tau levels in SH-SY5Y cells. SH-SY5Y cells were transfected with either the empty plasmid as a control or with the same plasmid carrying the Syk sequence for overexpression (Syk OX). Proteins expressed by transfected SH-SY5Y cells were analyzed by Western-blotting. Western-blots chemiluminescent signals were quantified and results were tested for normal distribution using the Shapiro-Wilk test. A subsequent Mann–Whitney test was used to test for statistical significance. a Representative Western blots are shown. b Level of Syk overexpression is in average 17.3 times higher than in control cells (p ≤ 0.0001, n = 18). c Level of tau phosphorylation at Y18 is 1.7 times higher in Syk overexpressing compared to control cells (p ≤ 0.01, n = 11). d Level of tau phosphorylation at S396/404 is 3 times higher in Syk overexpressing compared to control cells (p ≤ 0.0001, n = 17). e Level of total tau (DA9) is 4.1 times higher in Syk overexpressing compared to control cells (p ≤ 0.0001, n = 18). The error bars represent SEM