Fig. 1
From: Alzheimer’s disease pathological lesions activate the spleen tyrosine kinase
pSyk is increased in activated microglia and non-glial cells associated with Aβ-plaques in Tg APPsw and Tg PS1/APPsw mice. a Spatial distribution and cellular localization of activated/phosphorylated Syk were investigated in the cortex of 116 ± 13.5-week-old (avg. ± SEM) wild-type mice (n = 6) by triple-immunostaining of pSyk (Y525/526, green), microglia (Iba1, red) and astrocytes (GFAP, purple). Nuclei were stained with DAPI (blue). b Syk activation in wild-type animals was compared to age-matched Tg APPsw (n = 6) (b-c) and Tg PS1/APPsw littermates (n = 6) (d-e). Plaque-associated cortical areas (c, e) were compared to non-plaque-associated areas (b, d). Qualitative image analysis of orthogonal projections and 3D-image analysis (not-shown) revealed an increased pSyk burden in transgenic (b-e) compared to wild-type mice (a) and a colocalization of pSyk and Iba1 but not GFAP in Aβ-overexpressing animals (b-d). Large, non-glial spherical accumulations of pSyk were observed in plaque-associated areas (e). The scale bar represents 10 μm