Fig. 4

Peripheral injection of LPS promotes the influx of RBC-EVs crossing BBB and uptake of RBC-EVs by microglia. Images of brain slice with peripheral injection of LPS + DiI-EVs, LPS + PBS, saline + DiI-EVs or saline + PBS were labeled with DAPI to visualize the cells a Note that DiI-EVs can be only detected in LPS + DiI-EVs injected group at cerebral nuclei, cortex, interbrain, midbrain, substantia nigra (SN), but not at hippocampus. Scale bar, 10 μm. Graphs show numbers of DiI-EVs co-localized with DAPI in cerebral nuclei, cortex, interbrain, midbrain, substantia nigra and hippocampus in mouse with LPS + DiI-EVs, LPS + PBS, saline + DiI-EVs or saline + PBS administration b n = 3 independent animals in each group. Images of brain slice were co-labeled with Iba-1 and GFAP or labeled with MAP2 c Note that DiI-EVs co-localized with Iba-1. Scale bar, 10 μm. Images of brain slice were co-labeled with Iba-1 and GFAP d or labeled with MAP2 e Three-dimensional analysis of the co-localization within the outlined area is on the right. The xz and yz sections along the dashed lines on the xy image are shown. Note co-localization of the DiI-EVs with Iba-1 labeled microglia