Skip to main content
Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Transmission of α-synuclein-containing erythrocyte-derived extracellular vesicles across the blood-brain barrier via adsorptive mediated transcytosis: another mechanism for initiation and progression of Parkinson’s disease?

Fig. 1

The characterization of extracellular vesicles from packed red blood cells (RBC-EVs). SEC was used to separate EVs (peak 1) from low molecular weight protein contaminants [peak 2; co-eluted with BSA (66 kDa)]. The presence of (EV surface) proteins was monitored by UV absorbance at 280 nm (a), and the number of particles in each fraction was further measured by NTA (b). The total particle number of RBC-EVs (c) and the distribution of RBC-EVs (d) were measured in each peak by NTA (Nanosight NC300). Representative images of Western blots showing Alix and CD235a in RBC-EVs and RBC cell lysate e Three independently cultured and prepared samples of RBC lysate and EVs (50 μg of total proteins in each sample) were used for western blot analysis and were tested by immunoblot with antibodies against Alix (exosome marker) and CD235a (RBC marker). The α-syn level in RBC-EVs was measured using Luminex immunoassay (f), compared to the α-syn level in RBC cell lysates. Values are means ± S.E.M, n = 3

Back to article page

Acta Neuropathologica Communications

ISSN: 2051-5960

Contact us