Fig. 6

Schematic representation of the endosomal and exosomal pathways in diploid and DS neurons. Endocytosed material in the cell is transported by early endosomes and late endosomes/multivesicular bodies (MVBs) for either degradation in lysosomes or exosome secretion. The invagination of the MVBs membrane results in the formation of intraluminal vesicles (ILVs), which are released as exosomes into the extracellular space upon fusion of MVBs with the plasma membrane. Our findings show that in DS neurons with endosomal enlargement there is an enhanced exosome release regulated by the tetraspanin CD63