Fig. 4

Exosome secretion is enhanced by DS fibroblasts as compared to 2N controls. a Representative Western-blots and corresponding quantification showing the higher levels of the exosomal markers CD63, Alix and TSG101 in EVs isolated from the conditioned media of DS fibroblasts compared to 2N controls. The intensity of the bands was normalized to cell protein. b Elevated expression levels of CD63 and rab35 and (c) no differences in the levels of Alix and TSG101 in lysates of DS fibroblasts compared to 2N controls, as shown by the representative Western-blots and corresponding quantification. β-actin was blotted as an internal control for loading. Student t-test, n = 5 independent experiments (*p < 0.05; **p < 0.01)