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Table 3 Primer sequences of IDH1/IDH2, TERT promoter, and MGMT for Sanger sequencing

From: The frequency and prognostic effect of TERT promoter mutation in diffuse gliomas

  Prime  
Gene Forward Reverse Product (bp)
IDH1 5′-M13-GTA AAA CGA CGG CCA GTC GGT CTT CAG AGA AGC CA-3′ 5′-GCG GAT AAC AAT TTC ACA CAG GGC AAA ATC ACA TTA TTG C-3′ 180–190
IDH2 5′-GCT GCA GTG GGA CCA CTA TT-3′ 5′-TGT GGC GTT GTA CTG CAG AG-3′ 295–305
TERT 5′-M13-GTA AAA CGA CGG CCA GTC ACC CGT CCT GCC CCT TCA CCT T-3′
(M13: 5′-GTA AAA CGA CGG CCA GT-3′)
5′-GCA CCT CGC GGT AGT GG-3′ 300–310
MGMT 5′-TTT CGA CGT TCG TAG GTT TTC GC-3′ 5′-GCA CTC TTC CGA AAA CGA AAC G-3′ 80–90
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