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Table 2 Phenotypes of OSK-KI mice in comprehensive behavioral test battery

From: Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer’s disease: analysis of Osaka mutation-knockin mice

Tests Hetero-KI Homo-KI
(vs. Non-KI) (vs. Non-KI) (vs. Hetero-KI)
Somatic parameters    
 Body weight 1
 Body temperature
 Grip strength
 Wire-hanging time
Light/dark transition test    
 Anxiety 2
Open field    
 Exploratory locomotion
Elevated plus maze    
 Anxiety
Rotarod    
 Motor coordination 3 3
Hot plate    
 Pain sensitivity
Social interaction test (novel environment)
Social interaction test (Crawley’s version)
Startle Response/Prepulse inhibition    
 Sensorimotor gating 4 4
Porsolt forced swim    
 Immobility time (behavioral despair)
Barns maze    
 Spatial memory 5
Eight-arm radial maze    
 Working memory 6
T-maze forced alternation    
 Working memory
Gait analysis    
 Gait ataxia
Fear conditioning    
 Contextual fear memory
Tail suspension    
 Immobility time (behavioral despair)
Social interaction test (24-h homecage monitoring) 7
  1. Comprehensive behavioral test battery was performed on 8-month-old male mice as described previously [10]. The numbers of mice used in each test were n = 15–17 for non-KI, n = 16–20 for hetero-KI, and n = 12–16 for homo-KI except for the social interaction tests (novel environment and 24-h homecage monitoring) in which n = 7–9 for non-KI, n = 8–10 for hetero-KI, and n = 6–8 for homo-KI. ─, no significant difference was detected. ↓1, body weight of homo-KI was slightly but significantly (p < 0.05) lower than that of hetero-KI. ↓2, distance traveled in dark of homo-KI was slightly but significantly (p < 0.05) lower than that of hetero-KI. ↓3, latency to fall of hetero-KI was significantly (p < 0.05) lower than that of non-KI; latency to fall of homo-KI was also lower than that of non-KI, but the difference was not significant. ↓4 and ↑4, prepulse inhibition of hetero-KI was lower than those of non-KI and homo-KI, but the differences were not significant. ↑5, error to 1st of homo-KI was slightly but significantly (p < 0.05) lower (i.e. improved) than that of non-KI. ↑6, different arm choices in first 8 entries of homo-KI was slightly but significantly (p < 0.05) higher (i.e. improved) than that of non-KI. ↓7, activity level in dark of homo-KI was significantly (p < 0.05) lower than that of non-KI