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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer’s disease: analysis of Osaka mutation-knockin mice

Fig. 7

Effects of diazepam treatment on memory and Aβ pathology in OSK-KI mice. Diazepam (DZP) was orally administered to 6-month-old homo-KI mice at 2 μg/day for 2 months. (a) Spatial reference memory was examined at 8 months using the Morris water maze. Each point represents the mean latency of five trials per day ± SEM. *p < 0.05 versus non-KI. (b) Brain sections were stained with Aβ oligomer-specific 11A1 antibody. Photographs were taken from the PPtA of the cerebral cortex (CTX), hippocampal CA3 region (CA3), dentate gyrus (DG), and entorhinal cortex (EC). Arrowheads indicate Aβ accumulated within neurons. Scale bar = 30 μm. (c) Brain sections were stained with anti-synaptophysin antibody. Photographs were taken from the hippocampal CA3 region. Scale bar = 30 μm. Fluorescence intensities in a constant area were quantified. Each bar represents the mean ± SEM (n = 5 for each group). AU, arbitrary unit. (d) Brain sections were stained with anti-parvalbumin antibody. Photographs were taken from the dentate gyrus. Scale bar = 30 μm. Parvalbumin-positive GABAergic neurons in a constant area in the granular cell layer (GCL) of the dentate gyrus were counted. Each bar represents the mean ± SEM (n = 5 for each group)

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