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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Generation and characterization of new monoclonal antibodies targeting the PHF1 and AT8 epitopes on human tau

Fig. 6

Characterization of the novel tau antibodies in detecting biochemically sarkosyl-insoluble tau in human brain lysates from AD patients. Immunoblotting analysis of the sarkosyl-insoluble fraction from the temporal cortex of human AD cases (n = 3) and control cases (CTR; n = 2). Samples were biochemically fractionated as described in “Material and Methods.” Equal amounts of proteins (10 μg) from each sample was resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with each antibody indicated above blot, including total tau antibody 3026. The mobilities of molecular mass markers are shown on the left

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Acta Neuropathologica Communications

ISSN: 2051-5960

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