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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Generation and characterization of new monoclonal antibodies targeting the PHF1 and AT8 epitopes on human tau

Fig. 1

Specificity of novel tau antibodies raised against tau peptide p391–409 as determined by immunoblotting with recombinant tau proteins phosphorylated in vitro with p42 MAPK or GSK3β. WT, S396A and S404A 2 N/4R tau were incubated with p42 MAPK or GSK3β or without any kinase as described in “Material and Methods.” The proteins were resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with novel tau antibodies (as indicated for each blot) PHF2, PHF15, PHF17, PHF20 or PHF22. Similar blots were performed with previously characterized antibody PHF1 and total tau antibody H150. The mobilities of molecular mass markers are shown on the left

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