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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Mechanisms underlying extensive Ser129-phosphorylation in α-synuclein aggregates

Fig. 8

Effect of Ser129-phosphorylation on α-syn aggregate formation in a rat AAV-mediated α-syn overexpression model. Rats were sterotaxically injected with rAAV particles into the substantia nigra, and they expressed A53T mutant α-syn or A53T plus S129A double mutant α-syn. These rats were used in our previous study [18]. Information on the expression levels and toxicity is described in this paper [18]. Upper panels show the photomicrographs of rat striatum immunohistochemically stained with antibody specific to Ser129-phosphorylated α-syn and human total α-syn (LB509). We counted the number of α-syn-positive aggregates larger than 5 μm in diameter. Arrow heads indicate α-syn aggregates. Bar shows 100 μm. Graph shows quantitative analysis of striatal aggregates containing Ser129-phosphorylated α-syn or total α-syn. Data represent means ± SD and P values were estimated by one-way ANOVA with Bonferroni correction (*, P < 0.05; **, P < 0.01)

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