Fig. 5
ZIKV infection leads to changes in principal neural cell types in Ifnar1 knockout cultures. a and a i overlay F480-labelled microglia appear to engulf pyknotic nuclei (white arrows) and possibly also ZIKV-infected cells (yellow arrow). b, b overlay, c, c i overlay Some infected astrocytes appear relatively normal, with filamentous-appearing GFAP staining (b), whereas in other cases the filaments appear condensed (c). d i The majority of oligodendrocyte precursors were not infected and appeared normal. A proportion of PLP/DM20 positive oligodendrocytes was not infected and retained their myelin sheaths, e i whereas many of these cells were infected and appear morphologically abnormal with absent myelin sheaths (see Fig. 4). f, f i overlay Occasionally neurones were infected and had ZIKV +ve puncta in the cell body and in processes leading from the cell body. In infected neurons and oligodendrocytes, the nucleus often appeared to be displaced to the edge of the soma, as in this case