Fig. 2

ZIKV infects all principal CNS cell types. a-e Micrographs of infected neural cell types from Ifnar1 KO cultures at 24 or 72 hpi with ZIKV. a NeuN-labelled neurone (b) GFAP-labelled astrocyte; inset shows an example of an infected cell in which the glial fibrils appear condensed, ​(c) PLP/DM20-labelled oligodendrocyte (white arrow) with a fragmented myelin sheath (broken yellow arrow), below which there is normal-appearing myelin sheath (solid yellow arrow), (d) NG2-labelled OPCs and (e) F480-labelled microglial cell, all containing detectible levels of ZIKV. Bars: 10 μm. f-h Graphs showing densities of total (grey) and infected (black) cells in Ifnar1 knockout mouse cultures, treated with vehicle (f; mock), ZIKV at MOI 0.3 24 hpi (g) or ZIKV at MOI 3.0 72 hpi (h).​ In mock infected cultures a small number of cells appeared positive for ZIKV and represent false positives. Bars represent mean ± SD; n = 3 for all except ‘microglia MOI 0.3 24 hpi’ where n = 2