Fig. 1

ZIKV infection is limited by the type I interferon response in neural cell cultures. a-d Micrographs of infected cells in myelinating cultures from wild type (WT) CNS (a), Ifnar1 knockout (KO) CNS (b), WT PNS (c) and Ifnar1 KO PNS (d) infected with a multiplicity of infection (MOI) of 3.0 for 72 h. Bar: 100 μm. e, f, Graphs of total (grey bars) and infected (black bars) cell densities in mock infected and ZIKV infected CNS cultures at MOI and hours post infection (hpi) indicated. The percentage of infected cells is indicated above each bar. Compared to wild type cultures, more cells are infected in the absence of a type I interferon response. g, h Graphs of total and infected cell densities in PNS cultures. Note the different scale on the lower Y axis. Infection rates are very low even in the absence of a type I interferon response. There is a proportion of cells that are false positives, as indicated by black bars in the mock infected cultures. Bars represent mean ± SD; n = 3 for all except ‘WT CNS’ and ‘WT PNS mock’ where n = 2