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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: PLGF, a placental marker of fetal brain defects after in utero alcohol exposure

Fig. 1

Effects of in utero alcohol exposure on brain angiogenesis and expression of members of the VEGF/PLGF family from E20 embryos. a, b Effects of fetal alcohol exposure from GD15 to GD20 on the organization of cortical microvessels in control and alcohol-exposed animals. Brain microvessels were visualized by immunohistochemistry against CD31. Arrows indicate brain microvessels presenting a radial orientation in the control group. Note a loss of the radial organization in the alcohol-exposed group. I-VI: Cortical layers; CC: Corpus callosum. c Distribution of the orientation (angle classes) of cortical microvessels in the immature cortex from GD20 fetuses. Statistical analysis was performed using the χ2 test. d Quantification by Western blot of the effects of fetal alcohol exposure during the last gestational week on the cortical expression of CD31 at GD20. e-i Quantification by Western blot of VEGFA, PLGF, sVEGF-R1, mVEGF-R1 and VEGF-R2 protein levels in the cortex from control and alcohol-exposed groups. *p < 0.05 vs the control group using the unpaired t test. j Comparison by Western blot of the PLGF protein levels in the cortex and the placenta of E20 embryos from the control group. ***p < 0.001 vs the control group using the unpaired t test

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