Fig. 4

Strain-specific properties are retained after fixation. a Fixed hippocampi were isolated from PS19 mice at 12 weeks post injection with DS9 or 10. This tissue was homogenized and transduced into the original LM1 cell line. Fluorescence-activated cell sorting was used to isolate monoclonal cells into 96-well plates. Cells that stably propagated aggregates were amplified and characterized. b Confocal images of representative secondary cell strains derived from mice inoculated with DS9 or 10. Secondary strains displayed the same inclusion morphology as the original inoculum (nuclear speckles or a large juxtanuclear aggregate). See Fig. 3a for images of original strain morphology. c. Seeding activity was assessed for DS9 and 10, as well as secondary cell lines. Cell lysate from each line was transduced into biosensor cells and assessed for tau seeding activity after 24 hours (2 μg per well). Secondary strains showed similar seeding activity to the original inoculum. See Table 2 for additional information regarding the mice used in this study