Fig. 4
Age-dependent loss of MBP and P25α signals in striosomes (patches). a A coronal brain section (6 μm, paraffin embedded) from a 12 month-old A53T α-Syn mouse, stained with anti-MBP antibody. Black square shows area of interest. b Higher magnification of the area of interest from (a) in a consecutive section, double stained with anti-MBP (red) and anti-tyrosine hydroxylase (TH, green) antibodies. Scale bar, 100 μm. c Quantification of MBP signal inside striosomes. Bars represent mean ± SD of n = 4 A53T and control mice at 2, 8 and 12 months of age. *, < 0.05, one-way ANOVA. d Quantification of MBP signal in striosomes of Thy-1 α-Syn tg and age-matched control mouse brains determined at 2 and 8 months of age. Bars represent mean ± SD of n = 4 mice. *, < 0.05, one-way ANOVA. e Quantification of MBP signal in striosomes of 12 month-old 5XFAD and control mice. f Coronal brain sections containing rostral-dorsal striatum (6 μm, paraffin embedded) of 12 month-old A53T α-Syn mouse and age-matched control immunoreacted with anti-P25α antibody and showing a striosome. Scale bar, 10 μm. g Samples of high-speed supernatant (50 μg protein) obtained from whole Thy-1 α-Syn and control mouse brains at 2 and 10 months of age analyzed by Western blotting and immunoreacted with anti-P25α antibody. h Graph showing quantitation of blots obtained in (G) mean ± SD of n = 4 mice. *, < 0.05, one-way ANOVA