Fig. 2

Myelin proteins, membrane flotation and histology. a Western blot showing myelin proteins in preparations of purified myelin from A53T α-Syn and control mouse brains at 4–6 months of age (n = 5–6 mice). b Bars showing mean ± SD of the indicated myelin protein levels, in whole brain protein extracts, of A53T α-Syn at 12–14 months (n = 8–9 mice). Presented as a percent of control age-matched mouse brain, set at 100% (vertical line) *, < 0.05, one-way ANOVA. c Flotation assay showing the distribution of detergent-soluble myelin membrane particles into a 8–25% nycodenz gradient. Purified myelin preparations of A53T α-Syn and control mouse brains at 12 months of age, analyzed in parallel. Aliquots of gradient fractions analyzed by Western blotting using the specified antibodies. Representative blot of n = 3. 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase); myelin associated glycoprotein (MAG); myelin basic protein (MBP); myelin oligodendrocyte glycoprotein (MOG); proteolipid protein (PLP). d The caudate nucleus in coronally sectioned human brain hemisphere (100 μm), including striosomes (dark strips) and matrix (light staining) of a 90-year old female without PD (non-PD) and a 68-year-old female PD patient with advanced disease (neuropathological stage 5). Sections were stained for myelin using a modified Pal-Weigert method. Scale bar, 500 μm. Stained brain sections were provided by the Braak laboratory (University of Ulm, Ulm, Germany). e Coronal brain sections (6 μm, paraffin embedded) of A53T α-Syn and control mice at 12 months of age stained with Luxol Fast Blue/Periodic Acid Schiff, showing the corpus callosum (cc) and striatum (st). Scale bar, 500 μm