Fig. 1

Higher levels of phospholipids in purified myelin from neuronal α-Syn-expressing mouse brains. a ³¹P NMR spectra of a sample consisting of a chloroform/methanol extract of purified myelin obtained from a Thy-1 human wt α-Syn tg mouse brain. The ³¹P NMR spectra were obtained with a 500 MHz NMR spectrometer (Bruker, Germany) with a 5 mm broadband probe. The ³¹P chemical shifts were assigned according to standard phosphatidic acid (PA) and phosphatidylserine (PS), spiked into the sample and referenced to phosphatidylcholine (PC) pick at 0 ppm. b The area under the curves was calculated for samples of A53T α-Syn and control mouse brains and the relative amount of specific phospholipids was calculated using the MNova data processing program (Mestrelab Research); mean ± SD of n = 5 mouse brains; *, < 0.05, one-way ANOVA. phosphatidylethanolamine plasmalogen (PE-plasm); phosphatidylethanolamine (PE); sphingomyelin (SPH); phosphatidylinositol (PI); phosphatidylcholin (PC)