Fig. 5
From: Hydrocephalus due to multiple ependymal malformations is caused by mutations in the MPDZ gene
Confocal analyses performed on ependyma sections double stained with anti- MPDZ (green) and EMA (red) showed a lack of MPDZ positivity in foetus 1 with EMA immunoreactivity at the apex of ependymal lining (a, b and c) contrary to the control where multiple MPDZ dots (green) were strongly expressed at the apical and lateral sides of the ependyma cell membrane and in the underlying cytoplasm (d, e and f) Immature cells surrounding the aqueduct and rosettes were only immunoreactive for nestin (arrow) (g) compared to the control case in which the dentate gyrus (used as a positive control) contained multiple stem cells co-expressing nestin (green cytoplasmic) and PAX6 (red nuclear) (arrow) (h)