Fig. 6

Altered Calpain levels in sCJD. a Expression of Cathepsin family, Calpain 1 (Capn1), Calpain 2 (Capn2) and Calpain 4 (Capns1) in the cortical region of the tg340-PRNP129MM mouse model at 180 (clinical) days after inoculation with sCJD MM1 brain homogenates. Data were generated by RNA-sequencing analysis. Fold Change line at 1.5 indicates the threshold of significant regulations in the expression levels for these genes between control and sCJD MM1 inoculated mice. b Western-blot and densitometry analysis of Cathepsin S and Cathepsin D expression in the frontal cortex and cerebellum of control (n = 9), sCJD MM1 (n = 9) and sCJD VV2 (n = 9) cases. ANOVA test followed by post-test Tukey’s Multiple Comparison Test was used to compare the values from different groups. P values for the comparisons of the three groups are indicated in the figure:*p < 0.05; **p < 0.01