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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Altered Ca2+ homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease

Fig. 4

Abnormal lysosomes in sCJD are dependent on Calpain over activation. a TEM images indicating the presence of processes with autophagosomes and lysosomes, distorted and abnormal lysosomes and auto-lysosomes in neurons of the frontal cortex of sCJD cases. b and c Increased Calpain 1/2 activity by fluorimetric Calpain activity (c) without alterations on Calpain 1 levels (b) in PCC treated with the prion protein peptide (106–126). Inhibition of Calpain activity by MDL28170 treatment partially reverses (d) decrease on Lysotracker signal and decrease on cell viability (e) caused by prion protein peptide treatment. Unpaired t-test (95% CI) was used for the comparisons of the two groups. Data from PCC are obtained from three independent experiments. ANOVA test followed by post-test Tukey’s Multiple Comparison Test was used to compare the values from different groups. P values for the comparisons of the three groups are indicated in the figure:*p < 0.05; **p < 0.01; ***p < 0.001

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