Fig. 3

Neuronal localization of Calpains in sCJD. a Immunohistochemical staining of frontal cortex and cerebellum sCJD stained either with Calpain 1 (green) and Iba-1 or GFAP (red). Tissues were counterstained with DAPI (blue). b Immunohistochemical staining of cerebellum sCJD stained with Calpain 2 (green) and Cd68 (red). Tissues were counterstained with DAPI (blue). c Immunohistochemical staining of frontal cortex sCJD stained with Calpain 1 (green) and MAP2 (red). Calpains are mainly expressed in neurons as shown in the merged panels. d Western-blot analysis of solubility assays in control (n = 3) and sCJD MM1 cases (n = 3) by means of differential centrifugation in which cytoplasm fraction (S2), membrane fraction (S3) and insoluble fraction (S4) were obtained. Samples were developed for Calpain1 and PrP antibodies. . Unpaired t-test (95% CI) was used for the comparisons of the two groups. *p < 0.05; **p < 0.01; ***p < 0.001