Fig. 2

Regional and subtype-dependent expression and activation of Calpains in sCJD. a qPCR analysis of expression levels of Calpain 1 and Calpain 2 in the frontal cortex and cerebellum of control, sCJD MM1 and sCJD VV2 cases. b Western-blot and densitometry of Calpain 1 and Calpain 2 in the frontal cortex and cerebellum of control, sCJD MM1 and sCJD VV2 cases (n = 14/group). c Calpain activity by means of fluorometric assay based on the detection of cleavage of Calpain substrate Ac-LLY-AFC in the frontal cortex of control, sCJD MM1 and sCJD VV2 cases (n = 6/group). d Western-blot and densitometric analysis of Fodrin and N-terminal cleaved Calpain-1 in the frontal cortex of control, sCJD MM1 and sCJD VV2 cases (n = 6/group). ANOVA test followed by post-test Tukey’s Multiple Comparison Test was used to compare the values from different groups. P values for the comparisons of the three groups are indicated in the figure:*p < 0.05; **p < 0.01; ***p < 0.001