Skip to main content
Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination

Fig. 6

MS#30 and NMO#53 cause cytotoxicity to targeted cells via complement activation. PLP-eGFP slices were treated with rAb + HC (al) or rAb + C5-depleted HC (mx) for 8 h and then stained for MAC and C3d deposition, respectively. Deposits of MAC (red) or C3d (red) were detected along PLP-eGFP+ oligodendrocyte processes and co-localized with MS#30 rAb (blue) in MS#30 + HC treated slices (ad, mp; arrows); whereas in NMO#53 + HC treated slices (eh, qt), MAC was co-localized with NMO#53 rAb (blue), which specifically labels astrocytes and end-foot processes (arrows), but was absent from oligodendrocyte processes (arrow heads). MAC and C3d staining were notably increased in MS#30 + HC or NMO#53 + HC treated slices when compared to Iso + HC treated ones (il, ux). Scale bar: 25 μm

Back to article page