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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination

Fig. 2

MS#30 plus human complement (HC) causes robust loss of mature oligodendrocytes but not progenitors. Confocal images of PLP-eGFP in slices treated with 20 μg/ml MS#30 plus 10% (vol/vol) HC for 8 (a), 12 (b), 24 (c) and 48 h (d) and isotype control rAb (Iso) + HC for 48 h (e). Quantification of eGFP+ oligodendrocyte cell bodies in slices (f). Cell body count was normalized to control (Iso + HC treated) slice at 8 h. 25X (g, i) and 40X (h, j) objective confocal images of PLP-eGFP in slices treated with MS#30 alone for 24 and 48 h. 25X (k, m, o, q) and 40X (l, n, p, r) objective confocal images of PLP-eGFP slices treated with MS#30 alone or MS#30 + HC at indicated time and co-stained with Olig2 (red) and NG2 (blue). Oligodendrocyte progenitors were identified as Olig2+ NG2+ cells (arrows). Olig2+ NG2- cells were mature oligodendrocytes (arrow heads) and overlapped with PLP-eGFP (25X objective panels). Quantification of NG2+ Olig2+ oligodendrocyte progenitor cell number in slices (s). Cell count was normalized to control (Iso treated) slice at 24 h. Statistical analyses were performed by multiple unpaired Student’s t test for single comparison (f) or by two-way ANOVA for grouped comparisons (s). ***: p < 0.001, ****: p < 0.0001, ns: not significant, n = 4. Scale bars: 50 μm

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