Fig. 5

Age-dependent dysfunction of DAT expression and function in G2019S knock-in (KI) mice. Kinetic analysis of [³H]-DA uptake in synaptosomes (a, c), and Western blotting analysis of DAT protein levels (and representative blots) (b, d) were performed in the striata of 12-month-old (a, b) and 3-month-old (c, d) G2019S KI mice in comparison with age-matched WT controls. Values are expressed as mean ± SEM of n = 4 (uptake) or n = 3 (Western blotting) independent experiments performed in duplicate. Statistical analysis was performed using the Student t-test, two-tailed for unpaired data. *p < 0.05, different from WT