Fig. 8
From: The origin of Rosenthal fibers and their contributions to astrocyte pathology in Alexander disease
Mitotic abnormalities in astrocytes with Rosenthal fibers (RFs) in AxD mouse (a) and AxD human brains (b-f). a RFs (arrows in (a1)’) between metaphase chromosomes in a mitotic astrocyte in 1 week-old double mutant mouse. A1 Single optical slice of enlarged boxed area in (a). Double immunostaining for GFAP and Ki67, counterstaining with DAPI. Confocal microscopy. For animation of Z-stack of optical slices shown in (a) see Additional files 10 and 11: Movie 5 and 5a. b Astrocyte with misaligned metaphase chromosomes intermingled with RFs (arrows). H&E staining. c Area in brain stem with many eosin-positive RFs. Arrow indicates RF as intranuclear pseudoinclusion. H&E staining. d RFs (arrows) in astrocyte with several nuclei. (e) GFAP immunopositive area (arrow) in the nucleus of an astrocyte. e1 2D projection to 3D reconstruction of the cropped area from (e). Note that GFAP+ pseudoinclusion (arrow) is located in a deep invagination of nuclear envelope and is not superimposed on the nuclear profile (N). Double immunostaining for GFAP and vimentin, counterstaining with Nissl. Confocal microscopy. f Multinuclear astrocyte with RFs. Immunostaining for GFAP and counterstaining with DAPI and Nissl. Note many RFs in vicinity of micronuclei in F’. Confocal microscopy. Scale bars: 8 μm in (a); 6 μm in (b, d); 12 μm in (c); 4 μm in (e); 12 μm in (e1); 11 μm in (f)