Fig. 4
From: The origin of Rosenthal fibers and their contributions to astrocyte pathology in Alexander disease
Immunogold staining of RFs. a, b Double immunogold staining for alphaB-crystallin (18 nm particles) and GFAP (12 nm particles) in 1 year-old TG (a) and KI homozygous (b) mice. Note that every large and small electron dense profile of RFs contains small (GFAP) and large (alphaB-crystallin) gold particles indicating the presence of both antigens. c) Immunogold staining of RFs in subpial astrocytes for GFAP (12 nm particles). Note that large and small electron dense profiles are diffusely covered with gold particles. KI homozygous 1 year old mouse. a1, b1, b2, and (c1)–enlargements of boxed areas in (a), (b), and (c) respectively. Scale bars: 1 μm in (a),(c); 2 μm in (b)