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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Isolation of primary microglia from the human post-mortem brain: effects of ante- and post-mortem variables

Fig. 4

Microglia phenotype in relation to diagnosis and donor variables. a Fluorescence geometric means for CD45 and CD11b of microglia isolated from MS or control WM tissue. CD45 expression is significantly higher, CD11b expression does not reach significance (unpaired t test). Isolations performed using the previous method are denoted in red, those using the current method in blue, continued in following graphs. b-c Fluorescence geometric mean for CD45 and CD11b expression of microglia from WM and GM from non-demented control donors only. CD45 expression but not CD11b expression of WM microglia is increased compared to GM microglia (Mann-Whitney test). d-f Correlation plots of fluorescence geometric mean of CD45 expression by WM microglia show no significant correlation with CSF pH, PMD, or age (Pearson correlation). g-i Correlation plots of fluorescence geometric mean of CD11b expression by WM microglia show no significant correlation with CSF pH, PMD, or age (Pearson correlation). j-l Correlation plots of fluorescence geometric mean of CD45 expression by GM microglia show no significant correlation with CSF pH, PMD, or age (Pearson correlation). m-o Correlation plots of fluorescence geometric mean of CD11b expression by GM microglia shows a significant positive correlation with PMD, but not with CSF pH or age (Pearson correlation). ***p value < 0.001, ****p value < 0.0001

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