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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Isolation of primary microglia from the human post-mortem brain: effects of ante- and post-mortem variables

Fig. 2

Isolated microglia from post-mortem human CNS tissue are distinguishable from autologous macrophages. a FACS plot showing non-labeled (red) and far red cell tracker-labeled (blue) populations of CP-derived macrophages, CD11b/CD45 expression for both populations are shown in the FACS plot of the corresponding number. b FACS plot showing a non-labeled population of WM microglia, note the absence of cell tracker signal. c FACS plot showing a mixed population of cell tracker-labeled CP macrophages and non-labeled WM microglia, CP-derived macrophages are clearly separated by cell tracker labeling. d Contour plot showing the forward (FSC-A) and sideward (SSC-A) scatter distribution of non-labeled WM microglia (red) and cell tracker-labeled CP macrophages (blue), showing distinct population size and granularity for each group. e The same population of mixed cells as in C, showing CD11b and CD45 immunolabeling, showing increased staining for both markers in CP macrophages (blue) compared to WM microglia (red). f-g Quantification of the same cell tracker labeling strategy from seven brain donors shows that CD11b and CD45 geomean is increased in CP macrophages compared to WM microglia for all isolations (paired t-test). **p value < 0.01, ***p value < 0.001

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