Fig. 1

Microglia isolation method at a glance Depicted are the two similar methods through which microglia were isolated from post-mortem brain tissue. CNS samples were dissected from either occipital cortex (GM), corpus callosum (WM), or subcortical WM. Mechanical disruption of tissue was performed using scalpel (WM) or tissue sieve (GM). Dissociated tissue was then subjected to enzymatic digestion, using DNAse I and either collagenase I (previous method) or trypsin (current method), for 1 h and 45 min respectively. The resulting single cell suspension was subjected to gradient separation using Percoll. The glial cell fraction was extracted, washed, and subjected to CD11b + purification using magnetic beads. CD11b + cells were eluted by removing the column from the magnet and flushing the column