Fig. 3

Complement-mediated injury in CD59^+/+ and CD59^−/− astrocyte cultures and cerebellar slices. a. AQP4 and CD59 immunofluorescence in primary astrocyte cultures from neonatal CD59^+/+ and CD59^−/− rats. b. Complement-dependent cytotoxicity in astrocyte cultures following 2-h incubation with 5% human complement and indicated concentrations of AQP4-IgG (mean ± S.E.M., n = 4, * P < 0.01). c. AQP4, GFAP and C5b-9 immunofluorescence in cerebellar slice cultures from CD59^+/+ and CD59^−/− rats at 1 day after incubation with 5 μg/ml AQP4-IgG (or control-IgG) and 5% human complement. Fluorescence micrographs shown as low and high (boxed region) magnifications. Representative of 3 sets of slice culture studies