Skip to main content
Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Marked central nervous system pathology in CD59 knockout rats following passive transfer of Neuromyelitis optica immunoglobulin G

Fig. 1

Generation and characterization of CD59−/− rats. a. Schematic of CD59 gene deletion strategy showing deletion of 11 bp in exon 3, and PCR genotyping strategy. b. PCR genotype analysis following HpyCH4V restriction enzyme digestion, showing a single band at 269 bp for CD59−/− rats, two bands at 182 bp and 98 bp for CD59+/+ rats, and three bands for CD59+/− rats. c. Growth curves of CD59+/+, CD59+/− and CD59−/− rats (20 rats per group, differences not significant). d. Genotype distribution of 150 pups from breeding of CD59+/− rats. e. (left) CD59 immunofluorescence in blood smears. (right) Percentage erythrocyte lysis following complement activation (mean ± S.E.M., n = 6, **P < 0.01)

Back to article page