Skip to main content
Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: High plasticity of axonal pathology in Alzheimer’s disease mouse models

Fig. 6

Correlative two-photon in vivo imaging and FIB/SEM microscopy. (a, b), Optical single plane images taken at two different time points at the same region in layer I of the somatosensory cortex of the dE9 mouse, showing a Methoxy-X04-stained plaque (blue) and GFP-expressing processes. There is a loss of an AxD (arrow) in the space of 1 week. (c), Same region as in a, b taken ex vivo after the perfusion of the animal on day 8. This image was obtained by the combination of two optical single planes: one taken at the same z-level as in a, b and the other taken 4 μm above it, showing the NIRB marks (pseudocolored in yellow and orange) performed to locate the region of interest at the ultrastructural level. (d), Electron-micrograph picture from the last ultrathin section taken from the surface of the block that was further analyzed by FIB/SEM microscopy. The same NIRB marks (pseudocolored in yellow and orange) as in c can be seen. The plaque halo is pseudocolored in green. The rectangle shows the position and the x, z dimensions of the FIB/SEM stack that was obtained. The same rectangle is shown in a-c. The three dashed lines inside the rectangle show the perpendicular plane in the approximate region where images e-g were obtained by FIB/SEM. (e-g), Examples of FIB/SEM images that were taken in the z = 9.15 μm image stack (305 images of 30 nm thickness). Using Reconstruct software, the AxDs (green) and the microglial cell (red) that were present in the stack of images were segmented every 2 sections. (i), 3D visualization of the segmented elements (microglial cell: red, AxDs: green). There is an activated microglia cell in the region where the loss of a GFP-expressing AxD was observed. Scale bar (in g): 18.2 μm in a-c; 5.1 μm in d; 1.8 μm in e-h

Back to article page