Fig. 2
From: High plasticity of axonal pathology in Alzheimer’s disease mouse models
Three-dimensional reconstructions of AxDs. (a-f), Images obtained (over 148 days) of the same dystrophic axon expressing GFP (dys 1) in contact with an Aβ plaque stained with Methoxy-X04 (blue) in the supragranular layers of the somatosensory cortex of a dE9 mouse (two-photon microscopy). (g-l), Three-dimensional reconstructions of the AxD showed in panels a-f, respectively, using Imaris software. On day 91 (j) the degeneration of the distal part of the axon (arrowheads) begins. This degenerative process is completed in the successive days but the AxD remains at the end of the cut axon (k, l). Note that the AxD presents numerous changes in volume and shape. The existence of more than one swollen varicosity (asterisks in e show an example) and short axonal sprouting can be identified (arrows in b, c, f, h, i and l). Note that the dystrophic segment of the axon is shown in red and from this part the numerical AxD volume was calculated and plotted in Fig. 3a. The days shown refer to the number of days after day 0 (when imaging began). Scale bar (in l): 19.5 μm in a-l